PCR / RT -PCR Products

MAGSPIN-55

MAG SYBR® qPCR Kit is supplied as a ready-to-use mix containing all components required for amplification and detection of DNA in qPCR with exception of primers and template. The MAG SYBR® qPCR Kit is supplied as a 2X Master Mix with integrated antibody-mediated hot start Taq, SYBR® Green I fluorescent dye, MgCl2, dNTPs, ROX reference dye and stabilizers. MAG SYBR® qPCR Kit is intended for use on all real-time instruments and protocols. read more...

MAG02-II-400

The PCR Master Mix II (5X) is a ready-to-use mixture for all PCR applications. The mixes contain all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Mix II is supplied as a 5X concentrated ready-to-use mix containing 0.75 u Taq DNA polymerase, reaction buffer, 2 mM MgCl2 , 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture. Store at -20°C read more...

MAG02HS-II

The PCR Hot-Start Master Mix II (5X is a ready-to-use mixture for virtually all PCR applications. Hot Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to the standard DNA polymerase. The mixes contain Hot-Start Taq DNA polymerase and other components for PCR with the exception of template and primers. read more...

MAG02PFU-II

The PCR Pfu Master Mix II (5X) is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mixes contain Pfu DNA polymerase and other components for PCR with the exception of template and primers. Pfu DNA polymerase is isolated from the Pyrococus furiosus. The multifunctional thermostable enzyme possesses both of 5’ to 3’ DNA polymerase and 3’ to 5’ exonuclease activity which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. read more...

MAGSPIN-52

MAGTaq DNA Polymeraseis a thermostable DNA polymerase isolated and purified from E. coli strain that carries a plasmid with cloned Taq DNA polymerase. It is lacks 3'→5' proofreading activity and there is a 5'→3' exonuclease activity in the same polypeptide as the DNA polymerase.

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MAGSPIN-54

MAGHot Taq DNA Polymerase is complex mixture of a thermostable 94 kD Taq DNA Polymerase purified from E. coli recombinant strain expressing Thermus aquatiqus polymerase gene and specific monoclonal antibodies. MAGHot Taq DNA Polymerase is inactive under conditions of amplification reaction preparation. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to standard DNA polymerases. read more...

APSTP1000

MAGTaq DNA Polymerase is a recombinant thermo-stable DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With high DNA synthesis rate and thermo-stability, MAGTaq DNA Polymerase is suitable for general and specialized PCR applications.

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APSTK1000

APSMAGTaq SEMI-Taq DNA Polymerase is a specially blended enzyme mix containing SEMI-Taq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The SEMI-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. read more...

APSTF1000

The APSMAGTaq HiFi DNA Polymerase is a new genetic modified, recombinant DNA polymerase with 70 times higher fidelity during amplification than MAGTaq DNA polymerase, as well as very high elongation rate. Being highly thermostable, the HiFi DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The MAGTaq HiFi DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase. read more...

APSTP1100

The APSMAGTaq 5× PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5× concentrated master mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, 200 μM dNTP, enzyme stabilizer. read more...

APSTP1200

TThe APSMAGTaq 5× PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5× concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA Polymerase read more...

APSTP1260

The APSMAGTaq 5× Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The APSMAGTaq 5× Fluorescent PCR Master Mix is supplied as a 5× concentrated ready-to-use mixture containing all the essential ingredients for PCR with the exception of template and primers. In addition, the mixture contains two electrophoresis tracking dye (Orange G and Xylene cyanol FF), as well as a safer fluorescent DNA staining dye read more...

APSTQ1100

The APSMAGTaq Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (Q-PCR) except primers and templates. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcriptase reaction mixture. The APSMAGTaq Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. read more...

APSRI1000

RNAok RNase inhibitor




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APSRP1000

The APSMAGRT Reverse transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, a RNA dependent DNA polymerase capable of generating the first strand cDNA using RNA template.


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MAG02D-HS

The PCR Dye Hot-Start Master Mix (5X) is a ready-to-use mixture for virtually all PCR applications. Hot-Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to the standard DNA polymerase. read more...

MAG90P-250

Pfu DNA polymerase is isolated from the Pyrococcus furiosus. The multifunctional thermostable enzyme possesses both 5’ to 3’ DNA polymerase and 3’ to 5’ exonuclease activity, which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerases. Pfu DNA polymerase has a temperature optimum between 72℃ and 78℃, and remains more than 95% active following one hour incubation at 95℃.
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MAG03DN

100 mM dNTP (2′-deoxynucleoside 5′-triphosphate) Set consists of all four deoxynucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. The deoxynucleotides are suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT tailing reactions. The product is supplied as ready-to-use solutions. The 100 mM dNTP Set
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MAG03D

10 mM dNTP (2′-deoxynucleoside 5′-triphosphate) Mix consists of all four nucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 10 mM, in a solution of 0.6 mM Tris-HCl (pH 7.5). The 10 mM dNTP Mix is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT-tailing reactions. Protocol

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MAG02D-PFU

The PCR Dye Pfu Master Mix (5X) is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mixes contain Pfu DNA polymerase and other components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. Pfu DNA polymerase is isolated from the Pyrococus furiosus. The multifunctional thermostable enzyme possesses both of 5’ to 3’ DNA polymerase and 3’ to 5’ exonuclease activity which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. read more...

MAG01A

The APS LABS First stranded cDNA Synthesis Kit (AMV) is provided with all components required to perform first-strand cDNA synthesis. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) catalyzes the polymerization of DNA using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5’-3’ DNA polymerase activity, the enzyme also possesses partial RNase H activity, which breaks apart RNA:DNA hybrids. read more...

MAG01M

The APS LABS First stranded cDNA Synthesis Kit (MAGscript) is provided with all components required to perform first-strand cDNA synthesis. MAGscript Reverse Transcriptase (MAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which is a frequent choice for cDNA synthesis because of its ease of use and low intrinsic RNase H activity.
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MAG02

The APS LABS First stranded cDNA Synthesis Kit (SuperMAGscript) is provided with all components required to perform first-strand cDNA synthesis. SuperMAGscript Reverse Transcriptase (SuperMAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which lacks a 3’ to 5’ exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cDNA amplification. read more...

MAG012-A

The APS LABS Two-Step AMV RT-PCR Kit has all the components required to perform first-strand cDNA synthesis and second strand DNA amplification. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) can catalyze polymerization of DNA using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5’-3’ DNA polymerase activity, the enzyme also possess some RNaseH activity, which breaks apart RNA:DNA hybrids. It is used primarily for the synthesis of first and second strand complementary DNA (cDNA) and primer extensions. read more...

MAG012-M

The APS LABS Two-Step MMLV RT-PCR Kit is provided with all components required to perform first-strand cDNA synthesis and second strand DNA amplification. MAGscript Reverse Transcriptase (MAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which is a frequent choice for complementary DNA (cDNA) synthesis because of its ease of use and low intrinsic RNase H activity. In the cDNA synthesis step, RNA was reverse transcribed by SAMscript RTase to produce its cDNA. read more...

MAG012-SS

Two-Step SuperSAMscrip RT-PCR kit




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APSRP1100

The APSMAGRT One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT One-Step RT-PCR Kit provides the user an alternative of the lengthy two steps – first strand generation, amplification solution by using a single mixture, single tube, one step reaction.
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MAG01-II

The One-Step RT-PCR Kit II is designed for reverse transcription and PCR amplification of specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses MAGscript reverse transcriptase for first-strand cDNA synthesis and Hot Start Taq DNA polymerase for PCR amplification. MAGscript Reverse Transcriptase (MAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase read more...

MAG01-III

The One-Step RT-PCR Kit III is designed for reverse transcription and PCR amplification of specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses SuperMAGscript reverse transcriptase for first-strand cDNA synthesis and Hot Start Taq DNA polymerase for PCR amplification. SuperMAGscript Reverse Transcriptase (SuperMAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase), which lacks a 3’ to 5’ exonucleolytic proofreading read more...

MAG01-II-Plus

The APS LABS Two-Step MMLV RT-PCR Kit is provided with all components required to perform first-strand cDNA synthesis and second strand DNA amplification. MAGscript Reverse Transcriptase (MAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase) which is a frequent choice for complementary DNA (cDNA) synthesis because of its ease of use and low intrinsic RNase H activity. read more...

MAG01-Plus/A

he APS LABS Two-Step AMV RT-PCR Kit has all the components required to perform first-strand cDNA synthesis and second strand DNA amplification. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) can catalyze polymerization of DNA using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5’-3’ DNA polymerase activity, the enzyme also possess some RNaseH activity, which breaks apart RNA:DNA hybrids. read more...